產品名稱：U251（膠質瘤細胞）
產品品牌：ATCC
價格：詢價
上海研生生化試劑有限公司是一家專業經營ELISA 試劑盒、放免試劑盒、儀器耗材、科研細胞株的公司。 本產品U251，膠質瘤細胞相關詳細信息請。
產品說明：
Subculturing:
 Protocol:

1.  Remove and discard culture medium.

2.  Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.  Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.   Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.   Add appropriate aliquots of the cell suspension to new culture vessels.

6.   Incubate cultures at 37°C.

Subc*tion ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium renewal: 2 to 3 times per week

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